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For this, nodule structure and gene expression profiles—including the expression of genes associated with nodule development ( EFD , NF-YA1 ), (h)GSH biosynthesis ( GSH1 , GSHS , and hGSHS ), markers of defense responses ( PR1 , PR10 ), and nodule senescence markers ( TPP , Cyp15a )—were compared among untreated, BSO-treated, and BSO+GSH-treated roots with nodules (Figure 7)